Measurements by fluorescence spectroscopy are subject to many more instrumental and physical variables than absorption measurements. Due to these effects, the same sample may not give identical results with different instruments even if they are of similar design. Physical effects such as quenching or self-absorption may lead to departures from expected concentration/emission relationships over quite narrow concentration ranges. For these reasons it may be impossible to produce universal “absolute” reference materials, as certified values may be instrument dependent. Provided homogeneity and stability can be established, however, reference materials can be produced that allow users to monitor the performance of a spectrofluorometer over time and can also indicate the relative performance of different instruments. Starna fluorescence references are produced in an ISO17034 accredited production facility and calibrated in an ISO/IEC 17025 accredited calibration laboratory. Several of these references are cited in USP Chapter <853> for spectrofluorometer qualification. 

Spectral Response & Linearity - Starna Quinine Sulfate References 

Used to qualify the spectral response of a spectrofluorometer and can also, over a limited concentration range (up to 1 mg/l), be used as a linearity check. USP cited reference.   

More information

 

Sensitivity & Signal to Noise Ratio - Starna High Purity Water Reference  

 
This Reference Material uses Raman scattering to simulate the behaviour of fluorescent materials at very low concentrations and can be used to determine the Signal to Noise Ratio of a spectrofluorometer near its limit of detection and hence indicate its ultimate sensitivity. USP <853 > cited reference.

More information

 

Spectrofluorometer Performance and Wavelength Validation - Starna 6BF Series 

 

Used for spectrofluorometer wavelength qualification,  as a routine check of spectrofluorometer  performance and for inter-instrument and inter-laboratory comparisons. Six polymer blocks  containing seven organic fluorophores. Excitation range from 260 to 600 nm and emission range from 370 to 670 nm

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 Phosphorescence Decay Rate and Fluorescence Lifetime -  Starna Europium III Phosphorescence Reference

 
Used to confirm measurement of transient fluorescentsignals. Polymer block doped with Europium III. Decay rate and phosphorescence lifetime certified for gate times of  50 μS and 2 μS and a measurement delay time of 0.1 mS using excitation and emission wavelengths of 340 nm and615 nm respectively.

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Starna  Quantum Counter - Rhodamine  Solution Cell

 

Used by instrument manufacturers as a quantum counter  to determine spectral response factors to calculate ‘corrected’ fluorescence spectra. Designed to provide unity quantum conversion, so that the emitted fluorescence is directly proportional to the intensityof the exciting radiation. Usable range 220 nm – 580 nm. 

 

More information

 

 Starna Quantum Yield Reference – Dilute Rhodamine in Ethanol Cell 

 

 

Quantum yield of this solution is 1.0 at 25°C. Working excitation range 450 to 465 nm and emission range 550 to 650 nm. Blank-corrected absorbance of this solution is below thelevel recommended in the literature to avoid internal filter effects. Dilute Rhodamine 101 in ethanol in a sealed quartz cuvette with ethanol blank.  

More information

 

 Microplate Fluorometer Qualification - Starna Doped Glass Microplate References

 

For routine qualification of fluorescence-based plate readers for fluorescence intensity and well-to-well reproducibility.  96-well microplates containing inorganic fluorescent reference materials in glass matrix. The inorganic fluorophores are inherently stable, and do not suffer from photodegradation or bleaching.Formerly manufactured and supplied by Matech, USA. Available in top- and bottom-reading configurations. Range of excitation and emission wavelengths to match the user’s application. 

More information

 

Measurements by fluorescence spectroscopy are subject to many more instrumental and physical variables than absorption measurements. Due to these effects, the same sample may not give identical results with different instruments even if they are of similar design. Physical effects such as quenching or self-absorption may lead to departures from expected concentration/emission relationships over quite narrow concentration ranges. For these reasons it may be impossible to produce universal “absolute” reference materials, as certified values may be instrument dependent. Provided homogeneity and stability can be established, however, reference materials can be produced that allow users to monitor the performance of a spectrofluorometer and make inter-instrument and inter-laboratory comparisons. Starna fluorescence references are produced in an ISO17034 accredited production facility and calibrated in an ISO/IEC 17025 accredited calibration laboratory. Several of these references are cited in USP Chapter <853> for spectrofluorometer qualification. 

 

Quinine Sulfate References -Spectral Response and Linearity 

One of the first reference materials developed for fluorescence spectroscopy. Equivalent to NIST SRM 936a and cited in USP <853 >.

 

High Purity Water Reference- Sensitivity & Signal to Noise Ratio 

Used to determine the Signal to Noise Ratio of a spectrofluorometer near its limit of detection and hence indicate its ultimate sensitivity. USP <853 > cited reference.

 

Starna SF Series Doped Polymer References - Spectral Response Qualification

Five polymer blocks containing stable organic fluorophores.  Excitation range 280 to 700 nm and emission range 380 to 770 nm

 

Starna 6BF Series Doped Polymer References -Wavelength Validation and Performance Check

Six polymer blocks containing seven organic fluorophores. Excitation range 260 to 600 nm and emission range 370 to 670 nm.

 

Starna Europium III Reference - Phosphorescence Decay Rate and Fluorescence Lifetime  

Polymer block doped with Europium III. Decay rate and phosphorescence lifetime certified for gate times of 50 μS and 2 μS.  

 

Rhodamine Solution Cell - Quantum Counter

Used by instrument manufacturers to determine spectral response factors to calculate ‘corrected’ fluorescence spectra. Designed to provide unity quantum conversion,

 

Rhodamine in Ethanol Cell - Quantum Yield Reference

Quantum yield 1.0 at 25°C. Blank-corrected absorbance of this solution is below the level recommended in the literature to avoid internal filter effects. 

 

Starna Rare Earth Solution Cells -Spectrofluorometer Wavelength Qualification 

Cited in USP Chapter <853> for spectrofluorometer wavelength qualification. 

 

Doped Glass Microplate References - Microplate Fluorometer Qualification 

For qualification of fluorescence-based plate readers. 96-well microplates containing inorganic fluorescent reference materials in a glass matrix. Formerly supplied by Matech, USA.

 

 

Purpose

Used to determine the fluorescence quantum yield of a substance. 

Description and Discussion 

Solution of Rhodamine 101 in ethanol, sealed by heat fusion in to a high quality far-UV fluorometer cell, and supplied with an ethanol blank cell
The fluorescence quantum yield of a sample (ΦF) is the ratio of photons absorbed to photons emitted through fluorescence. This reference has a quantum yield of 1.0 at 25°C. By measuring the fluorescence emission of a sample with similar absorbance characteristics as the reference, and under the same conditions of measurement, the quantum yield of the sample can be determined.
The working excitation range is 450 to 465 nm and emission range 550 to 650 nm.
The blank-corrected absorbance of this solution is below the level recommended in the literature to avoid internal filter effects.  

How to Order 

  Catalogue Number
Starna Rhodamine 101 Quantum Yield reference  RM-RH101       

 

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DATASHEET DOWNLOAD 

Measurements by fluorescence spectroscopy are subject to many more instrumental and physical variables than absorption measurements. Due to these effects, the same sample may not give identical results with different instruments even if they are of similar design. Physical effects such as quenching or self-absorption may lead to departures from expected concentration/emission relationships over quite narrow concentration ranges. For these reasons it may be impossible to produce universal “absolute” reference materials, as certified values may be instrument dependent. Provided homogeneity and stability can be established, however, reference materials can be produced that allow users to monitor the performance of a spectrofluorometer over time and can also indicate the relative performance of different instruments. Starna fluorescence references are produced in an ISO17034 accredited production facility and calibrated in an ISO/IEC 17025 accredited calibration laboratory. Several of these references are cited in USP Chapter <853> for spectrofluorometer qualification. 

Spectral Response & Linearity - Starna Quinine Sulfate References 

Used to qualify the spectral response of a spectrofluorometer and can also, over a limited concentration range (up to 1 mg/l), be used as a linearity check. USP cited reference.   

More information

 

Sensitivity & Signal to Noise Ratio - Starna High Purity Water Reference  

 
This Reference Material uses Raman scattering to simulate the behaviour of fluorescent materials at very low concentrations and can be used to determine the Signal to Noise Ratio of a spectrofluorometer near its limit of detection and hence indicate its ultimate sensitivity. USP <853 > cited reference.

More information

 

Spectrofluorometer Performance and Wavelength Validation - Starna 6BF Series 

 

Used for spectrofluorometer wavelength qualification,  as a routine check of spectrofluorometer  performance and for inter-instrument and inter-laboratory comparisons. Six polymer blocks  containing seven organic fluorophores. Excitation range from 260 to 600 nm and emission range from 370 to 670 nm

More information

 

Phosphorescence Decay Rate and Fluorescence Lifetime -  Starna Europium III Phosphorescence Reference

 
Used to confirm measurement of transient fluorescentsignals. Polymer block doped with Europium III. Decay rate and phosphorescence lifetime certified for gate times of  50 μS and 2 μS and a measurement delay time of 0.1 mS using excitation and emission wavelengths of 340 nm and615 nm respectively.

More information

 

Starna  Quantum Counter - Rhodamine  Solution Cell

 

Used by instrument manufacturers as a quantum counter  to determine spectral response factors to calculate ‘corrected’ fluorescence spectra. Designed to provide unity quantum conversion, so that the emitted fluorescence is directly proportional to the intensityof the exciting radiation. Usable range 220 nm – 580 nm. 

 

More information

 

Starna Quantum Yield Reference – Dilute Rhodamine in Ethanol Cell 

 

 

Quantum yield of this solution is 1.0 at 25°C. Working excitation range 450 to 465 nm and emission range 550 to 650 nm. Blank-corrected absorbance of this solution is below thelevel recommended in the literature to avoid internal filter effects. Dilute Rhodamine 101 in ethanol in a sealed quartz cuvette with ethanol blank.  

More information

 

Microplate Fluorometer Qualification - Starna Doped Glass Microplate References

 

For routine qualification of fluorescence-based plate readers for fluorescence intensity and well-to-well reproducibility.  96-well microplates containing inorganic fluorescent reference materials in glass matrix. The inorganic fluorophores are inherently stable, and do not suffer from photodegradation or bleaching.Formerly manufactured and supplied by Matech, USA. Available in top- and bottom-reading configurations. Range of excitation and emission wavelengths to match the user’s application. 

More information

 
 
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PRODUCT ENQUIRY

 

user
FURTHER PRODUCT INFORMATION 
& HOW TO ORDER    

 

This Reference Material can be used to qualify the Signal to Noise Ratio of a spectrofluorometer near the limit of detection and hence indicate its ultimate sensitivity.
Traditionally, the performance of spectrofluorometers has been tested using dilute solutions of known fluorophores , but to use this approach at or near the detection limit of a high-performance instrument is impractical because of the very low sample concentrations required.
An alternative approach is to use the phenomenon of Raman scattering.  Because of the very small number of photons involved in Raman scattering, the signal levels are very low and comparable to those produced by very low analyte concentrations in normal fluorescence measurements. Pure water exhibits a Raman shift of tens of nanometers when excited by UV or visible radiation, so the Raman peak can be readily identified.

 

Fluorescence Intensity Correction Reference - Ultra-pure Water

  

Typical spectra are provided with excitation at 350nm and 500nm :

 

Raman

The signal –to-noise ratio may be calculated from these spectra using the instrument software.           
Note that the calculated ratio will depend on the calculation protocol employed by the instrument manufacturer and therefore  'compliance to specification' limits will be specific to the spectrofluorometer type under test.                           

 

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO/IEC 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.