User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry





This Reference Material can be used to qualify the wavelength calibration, in the ultraviolet and visible regions of the spectrum, of spectrophotometers with spectral bandwidths of 3 nm or less. It is accepted for this purpose by most Pharmacopoeias and Standardisation Bodies, including:

European Pharmacopoeia
Deutsches Arzneibuch (German Pharmacopoeia)
United States Pharmacopoeia
American Society for Testing and Materials
Therapeutic Goods Administration (Australia)
British Pharmacopoeia

This reference comprises Holmium oxide (4% m/v) in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette.
When prepared in perchloric acid, holmium oxide gives a spectral scan containing a series of 14 characteristic and well-defined peaks covering the wavelength range from 240 to 650 nm*.




Approximate peak wavelength values (in nm) are:
208*, 219*, 233*, 241, 250, 278, 288, 334, 346, 361, 385, 417, 451, 468, 485, 537, 641 (*Available on request)

*For instruments of 1 nm SBW, or less, this range can be extended to 200 nm - 650 nm.



A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.