User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry

 
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PRODUCT
ENQUIRY

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FURTHER PRODUCT INFORMATION 
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This Reference Material can be used to qualify the Spectral Bandwidth, in the ultra-violet region of the spectrum (265 nm – 270 nm), of spectrophotometers with spectral bandwidths of 3 nm or less It is accepted for this purpose by most Pharmacopoeias and Standardisation Bodies, including:

European Pharmacopoeia
Deutsches Arzneibuch (German Pharmacopoeia).
United States Pharmacopoeia
American Society for Testing and Materials
Therapeutic Goods Administration (Australia)
British Pharmacopoeia

This reference comprises two far UV quartz cells, one filled with a 0.020% v/v solution of toluene in hexane and the other a blank, filled with hexane only. Both have been permanently sealed by heat fusion.

When scanned in an ultraviolet spectrophotometer, the spectrum shows a maximum absorbance at 269 nm and a minimum at 267 nm. The ratio of the peak maximum at 269 nm to the minimum at 267 nm gives a measure of the resolution (Spectral Band Width, SBW) of the instrument.

 

RM-TX B K

Toulene-in-Hexane

The ratio is certified at a SBW of 1.0 nm.

 

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO 17034 (4001) as a Reference Material producer, and ISO/IEC 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.