These cell-based reference materials are designed for the qualification of HPLC UV detectors that can accept conventional spectrophotometer cells, e.g. Waters.

Erbium Oxide Solution – Wavelength Qualification

UV-Vis - Micro-Plate Reader Liquid Filter Reference Plate 

Erbium oxide in perchloric acid gives a spectral scan containing 9 well defined peaks from 230 nm to 660 nm. In typical HPLC DAD qualification protocols (e.g Waters) the three peaks at 255, 379 and 522 nm are used for wavelength qualification.

 

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Propyl Parabenz Solution – Photometric Linearity Qualification 

 HPLC Linearity Reference – Propyl Parbenz Plate

Heat sealed cells containing Propyl Parabenz solution in methanol. Five concentrations between 5 mg/l and 30 mg/l. When measured against the supplied methanol blank at 257 nm, the graph of absorbance against concentration is linear.

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This material can be used to qualify the wavelength scale of HPLC Diode Array detectors that can accept a 10 mm path length spectrophotometer cell in the sample position.

The use of Erbium oxide solvated in perchloric acid for the validation of the wavelength scale of HPLC Diode array detectors is well documented. When prepared in perchloric acid, erbium oxide gives a spectral scan containing a series of 9 characteristic and well-defined peaks covering the wavelength range from 230 nm to 660 nm. In typical HPLC validation protocols (e.g Waters) the three peaks at 255, 379 and 522 nm are used.

RM-ER B K 1

 

Erbium-liquid

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO Guide 34 (4001) as a Reference Material producer, and ISO 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials. 

 
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This material can be used to verify the photometric linearity of HPLC UV detectors (that can accommodate conventional UV cells) in compliance with published with HPLC qualification protocols

The use of Propyl Parabenz solutions in methanol for the validation of HPLC DAD detector linearity is well documented. Preparing these solutions in the laboratory can have hazards due to the volatility and toxicity of the methanol. Permanently heat sealed cells effectively isolate the operator fromthese problems.

The spectrum of propyl parabenz, when prepared in methanol, has an absorbance peak at 257 nm. Within the concentration range 5-30 mg/l, the apparent absorbance of a series of concentrations measured at these peaks will be a linear function of concentration.

Absorbance values are certified at 257 nm for concentrations of 5, 10, 20, 25 and 30 mg/l, measured against the supplied methanol blank.

RM-PPB B K

 

Propyl-Parabenz-Linearity

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO Guide 34 (4001) as a Reference Material producer, and ISO 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.