This material can be used to qualify the wavelength scale of HPLC Diode Array detectors that can accept a 10 mm path length spectrophotometer cell in the sample position.
Description and Discussion
Erbium Oxide (1% m/v) in 10% v/v perchloric acid, permanently sealed by heat fusion into a 10 mm far UV quartz cuvette.
The use of Erbium oxide solvated in perchloric acid for the validation of the wavelength scale of HPLC Diode array detectors is well documented. When prepared in perchloric acid, erbium oxide gives a spectral scan containing a seriesof 9 characteristic and well-defined peaks covering thewavelength range from 230 nm to 660 nm.
Approximate peak wavelengths (in nm) are:255, 364, 379, 405, 449, 487, 522, 541, 652.
In typical HPLC validation protocols (e.g Waters) the threepeaks at 255, 379 and 522 nm are used.
Note: The above values are for guidance only. Because the absorption bands are asymmetric, measured values will bespectral bandwidth dependent. The Calibration Certificateaccompanying each Starna Erbium Reference gives actual values measured at bandwidths of 1.00 and 5.00 nm, andonly these certified values should be used for instrument qualification. On request, Starna can provide certifiedvalues at other wavelengths and bandwidth values.
Erbium perchlorate reference cell