User Guide

For an introduction to the application and use of Certified Reference Materials in absorption spectrophotometry

 
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This material can be used to verify the photometric linearity of HPLC UV detectors (that can accommodate conventional UV cells) in compliance with published with HPLC qualification protocols

The use of Propyl Parabenz solutions in methanol for the validation of HPLC DAD detector linearity is well documented. Preparing these solutions in the laboratory can have hazards due to the volatility and toxicity of the methanol. Permanently heat sealed cells effectively isolate the operator fromthese problems.

The spectrum of propyl parabenz, when prepared in methanol, has an absorbance peak at 257 nm. Within the concentration range 5-30 mg/l, the apparent absorbance of a series of concentrations measured at these peaks will be a linear function of concentration.

Absorbance values are certified at 257 nm for concentrations of 5, 10, 20, 25 and 30 mg/l, measured against the supplied methanol blank.

RM-PPB B K

 

Propyl-Parabenz-Linearity

A Certificate of Calibration and Traceability and full instructions for use are provided with each Reference Material. Starna Scientific is accredited to both ISO Guide 34 (4001) as a Reference Material producer, and ISO 17025 (0659) as a Calibration Laboratory for optical reference measurements. Starna offers a Lifetime Guarantee on all its Reference Materials.