Purpose
This reference uses Starna’s proven and universally accepted UV and visible reference materials for the qualification of 96-well plate readers.
Description and Discussion
This device contains six Starna liquid CRMs in a black anodised aluminium microplate, manufactured to the Society of Biomolecular Screening (SBS) dimensional standards for a 96-well plate. The heat sealed, liquid filled cell formatutilises 5 mm path length cells that have a unique (patent applied for) bubble trap to minimise measurement errors. Potassium dichromate solution is used as an absorbance reference, and holmium oxide solution as a wavelength reference. These references are accepted by Pharmacopoeias and Standardisation Bodies as evidence of instrument qualification. Full details, spectra, etc of the individual reference materials may be found in the appropriate Starna data sheets.
The standard product has the following configuration of reference position and CRM:
| Position | Reference |
|
C2 - H2 |
Potassium dichromate 0.001M perchloric acid blank |
|
C4 - H4 |
Potassium dichromate 40mg/l in 0.001M perchloric acid |
|
C6 - H6 |
Potassium dichromate 80mg/l in 0.001M perchloric acid |
|
C8 - H8 |
Potassium dichromate 120mg/l in 0.001M perchloric acid |
|
C10 - H10 |
Potassium dichromate 160mg/l in 0.001M perchloric acid |
|
C12 - H12 |
Holmium oxide (8%) in 10% perchloric acid |
Absorbance values for the potassium dichromate references are certified at 235, 257, 313, & 350 nm and range from approximately 0.1 A to 1.0 A. The Holmium solution has 14 certified peak values from 240 nm to 640 nm.
Any standard format Starna sealed liquid CRM is available in the Starna microplate format. These include references for wavelength accuracy, absorbance accuracy, stray light and resolution. Please contact Starna for more information.